Hotspot

psaA-ycf3

LSC · 41484-42144 · 661 bp

Back to Cleistogenes

Pi 0.00055

MarkerSeek score 48.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 661
variable_sites: 2
pi: 0.00055
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GGGGCCCTTAGCTATTGTTC	TATAGCGCTTACTCCGGGAA	55.0	50.0	58.0	58.0	854-857 bp	1.0	80.2
psaA-ycf3_p2	GGGGCCCTTAGCTATTGTTC	GCGCTTACTCCGGGAAATTA	55.0	50.0	58.0	58.1	850-853 bp	1.0	79.9
psaA-ycf3_p3	GTCTGGCCCATTCCTCAAAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.1	58.0	819-822 bp	1.0	79.8
psaA-ycf3_p4	GTCTGGCCCATTCCTCAAAA	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.1	58.1	815-818 bp	1.0	79.4
psaA-ycf3_p5	CTGGCCCATTCCTCAAAAGA	TATAGCGCTTACTCCGGGAA	50.0	50.0	57.8	58.0	817-820 bp	1.0	78.6