Hotspot

petN-trnC-GCA

LSC · 17455-18302 · 848 bp

Back to Cleistogenes

Pi 0.00278
MarkerSeek score 53.40
Primer available yes
Label petN-trnC-GCA

Feature Metadata

label_name
petN-trnC-GCA
feature_type
igs
region
LSC
length_bp
848
variable_sites
7
pi
0.00278
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnC-GCA_p1 AGTCCACTTCTCCCCCATAC AGTTCAAATCTGGGTGCCG 55.0 52.6 58.1 58.1 891-958 bp 1.0 75.2
petN-trnC-GCA_p2 GTCCACTTCTCCCCCATACT AGTTCAAATCTGGGTGCCG 55.0 52.6 58.1 58.1 890-957 bp 1.0 75.2
petN-trnC-GCA_p3 AGCCCAAGCGAGACTTACTA AGTTCAAATCTGGGTGCCG 50.0 52.6 58.2 58.1 832-899 bp 1.0 75.0
petN-trnC-GCA_p4 AGTCCACTTCTCCCCCATAC CCAGTTCAAATCTGGGTGCC 55.0 55.0 58.1 59.1 893-960 bp 1.0 74.8
petN-trnC-GCA_p5 GTCCACTTCTCCCCCATACT CCAGTTCAAATCTGGGTGCC 55.0 55.0 58.1 59.1 892-959 bp 1.0 74.8