Hotspot

trnT-GGU-trnE-UUC

LSC · 14276-14814 · 539 bp

Back to Cleistogenes

Pi 0.00297
MarkerSeek score 48.50
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
539
variable_sites
6
pi
0.00297
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 AGCCCTTTTAACTCAGTGGT GCTGCCTCCTTGAAAGAGAG 45.0 55.0 56.7 58.3 659-664 bp 1.0 68.0
trnT-GGU-trnE-UUC_p2 AGCCCTTTTAACTCAGTGGT TGTCCTGAACCACTAGACGA 45.0 50.0 56.7 57.7 638-643 bp 1.0 67.8
trnT-GGU-trnE-UUC_p3 AGGCATAAGTCATCGGTTCA GCTGCCTCCTTGAAAGAGAG 45.0 55.0 56.6 58.3 622-627 bp 1.0 67.8
trnT-GGU-trnE-UUC_p4 AGGCATAAGTCATCGGTTCA TGTCCTGAACCACTAGACGA 45.0 50.0 56.6 57.7 601-606 bp 1.0 67.7
trnT-GGU-trnE-UUC_p5 TGGTAAGGCATAAGTCATCGG GCTGCCTCCTTGAAAGAGAG 47.6 55.0 57.5 58.3 627-632 bp 1.0 67.0