Hotspot

matK-trnK-UUU

LSC · 3224-3898 · 675 bp

Back to Cleistogenes

Pi 0.000808

MarkerSeek score 49.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 3
pi: 0.000808
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TTCCTGAAAGAGAAGTGGGT	GTCCGGTGTAATTCCATCGA	45.0	50.0	56.0	57.4	825-851 bp	1.0	61.8
matK-trnK-UUU_p2	TTCCTGAAAGAGAAGTGGGT	CGTCCGGTGTAATTCCATCG	45.0	55.0	56.0	58.8	826-852 bp	1.0	60.2
matK-trnK-UUU_p3	TTCCTGAAAGAGAAGTGGGT	TTCAATGTTTTGCCAGGAGC	45.0	45.0	56.0	57.2	845-871 bp	1.0	59.9
matK-trnK-UUU_p4	TTCCTGAAAGAGAAGTGGGT	TGGGTTGCTAACTCAATGGT	45.0	45.0	56.0	57.0	767-793 bp	1.0	59.0
matK-trnK-UUU_p5	TTCCTGAAAGAGAAGTGGGT	TCAATGGTAGAGTACTCGGCT	45.0	47.6	56.0	58.0	755-781 bp	1.0	58.7