Hotspot

psaC-ndhE

SSC · 114472-115043 · 572 bp

Back to Cleisostoma

Pi 0.0344

MarkerSeek score 75.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 572
variable_sites: 67
pi: 0.0344
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GGACAAGCGGATTCACATCT	GGTGCAATTACTATGGCCCA	50.0	50.0	58.0	57.9	729-1279 bp	0.363636	60.9
psaC-ndhE_p2	AGCTCGAACACATTGGGTAC	GGTGCAATTACTATGGCCCA	50.0	50.0	57.9	57.9	613-1163 bp	0.363636	60.7
psaC-ndhE_p3	TATACCAGAAAAGCCCGTGC	GGTGCAATTACTATGGCCCA	50.0	50.0	58.0	57.9	912-1462 bp	0.363636	60.6
psaC-ndhE_p4	GCTCGAACACATTGGGTACA	GGTGCAATTACTATGGCCCA	50.0	50.0	58.2	57.9	612-1162 bp	0.363636	59.9
psaC-ndhE_p5	TTGGACAAGCGGATTCACAT	GGTGCAATTACTATGGCCCA	45.0	50.0	57.8	57.9	731-1281 bp	0.363636	59.5