Hotspot

trnL-UAG-ccsA

SSC · 111732-111872 · 141 bp

Back to Cleisostoma

Pi 0.0485

MarkerSeek score 80.30

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 141
variable_sites: 26
pi: 0.0485
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	TGCTCTAAGGTCGCAAATATCA	50.0	40.9	57.9	57.6	215-268 bp	1.0	71.8
trnL-UAG-ccsA_p2	AAATTGGTAGACACGCTGCT	TGCTCTAAGGTCGCAAATATCA	45.0	40.9	57.8	57.6	222-275 bp	1.0	71.3
trnL-UAG-ccsA_p3	GGTAGACACGCTGCTCTTAG	TGCTCTAAGGTCGCAAATATCA	55.0	40.9	57.8	57.6	217-270 bp	1.0	71.2
trnL-UAG-ccsA_p4	GTAGACACGCTGCTCTTAGG	TGCTCTAAGGTCGCAAATATCA	55.0	40.9	57.8	57.6	216-269 bp	1.0	71.2
trnL-UAG-ccsA_p5	AATTGGTAGACACGCTGCTC	TGCTCTAAGGTCGCAAATATCA	50.0	40.9	58.3	57.6	221-274 bp	1.0	70.6