Hotspot

ycf1-rpl32

SSC · 110557-110966 · 410 bp

Back to Cleisostoma

Pi 0.145

MarkerSeek score 73.00

Primer available yes

Label ycf1-rpl32

Feature Metadata

label_name: ycf1-rpl32
feature_type: igs
region: SSC
length_bp: 410
variable_sites: 132
pi: 0.145
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rpl32_p1	GCTTCCTTCGTAACTCCAGG	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.0	57.9	1276-1639 bp	0.909091	87.3
ycf1-rpl32_p2	TAGAGTTCAACCCCCGTTCT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1322-1692 bp	0.727273	79.9
ycf1-rpl32_p3	TCATAGAGTTCAACCCCCGT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.1	57.9	1325-1695 bp	0.727273	79.7
ycf1-rpl32_p4	GCTTCCTTCGTAACTCCAGG	GAAACTTTTTGACTGCCCGG	55.0	50.0	58.0	57.9	919 bp	0.090909	42.5
ycf1-rpl32_p5	TAGAGTTCAACCCCCGTTCT	GAAACTTTTTGACTGCCCGG	50.0	50.0	58.0	57.9	965 bp	0.090909	42.4