Hotspot

clpP-psbB

LSC · 73176-73794 · 619 bp

Back to Cleisostoma

Pi 0.0334

MarkerSeek score 87.70

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 619
variable_sites: 64
pi: 0.0334
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 87.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CAACCAGAAACCAGAGCTGT	50.0	50.0	58.0	58.0	716-984 bp	1.0	89.8
clpP-psbB_p2	CTTTTGGAACACCAATGGGC	CAACCAGAAACCAGAGCTGT	50.0	50.0	57.8	58.0	662-930 bp	1.0	88.7
clpP-psbB_p3	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	638-906 bp	1.0	88.7
clpP-psbB_p4	ACCCAAACTGCATCTTCCTC	CAACCAGAAACCAGAGCTGT	50.0	50.0	57.8	58.0	702-970 bp	1.0	88.1
clpP-psbB_p5	TCCTCTCCAGGATTCCGAAA	CAACCAGAAACCAGAGCTGT	50.0	50.0	57.8	58.0	687-955 bp	1.0	87.9