Hotspot

trnK-UUU-rps16

LSC · 4455-4928 · 474 bp

Back to Cleisostoma

Pi 0.0276
MarkerSeek score 71.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
474
variable_sites
45
pi
0.0276
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TAAGAAGGGAAGCTCGTTGC TCGATCCCGAAGAGAAGGAA 50.0 50.0 57.9 57.9 1028-1124 bp 1.0 90.1
trnK-UUU-rps16_p2 TCGCAACCCAATCACTCTTT TCGATCCCGAAGAGAAGGAA 45.0 50.0 57.7 57.9 1238-1335 bp 0.818182 80.3
trnK-UUU-rps16_p3 TTCGCAACCCAATCACTCTT TCGATCCCGAAGAGAAGGAA 45.0 50.0 57.7 57.9 1239-1336 bp 0.818182 80.3
trnK-UUU-rps16_p4 TTTCGCAACCCAATCACTCT TCGATCCCGAAGAGAAGGAA 45.0 50.0 57.7 57.9 1240-1337 bp 0.818182 80.3
trnK-UUU-rps16_p5 ATACCCCGGAGACAAAGAGT TCGATCCCGAAGAGAAGGAA 50.0 50.0 57.8 57.9 1430 bp 0.090909 40.5