Hotspot

matK-trnK-UUU

LSC · 3285-4417 · 1133 bp

Back to Cleisostoma

Pi 0.0368

MarkerSeek score 82.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1133
variable_sites: 131
pi: 0.0368
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TCCTGAAGGAGTAGCGGATA	GATCCGGGTTGCTAACTCAA	50.0	50.0	57.0	57.6	1042-1380 bp	1.0	80.5
matK-trnK-UUU_p2	TCCTGAAGGAGTAGCGGATA	TTGATCCGGGTTGCTAACTC	50.0	50.0	57.0	57.6	1044-1382 bp	1.0	80.5
matK-trnK-UUU_p3	TCCTGAAGGAGTAGCGGATA	TCCGGGTTGCTAACTCAATG	50.0	50.0	57.0	57.9	1040-1368 bp	0.909091	79.1
matK-trnK-UUU_p4	TCCTGAAGGAGTAGCGGATA	CGGGTTGCTAACTCAATGGT	50.0	50.0	57.0	58.2	1038-1366 bp	0.909091	78.6
matK-trnK-UUU_p5	TCCTGAAGGAGTAGCGGATAT	TCCGGGTTGCTAACTCAATG	47.6	50.0	57.5	57.9	1040-1368 bp	0.909091	75.3