Hotspot

psbA-trnK-UUU

LSC · 1159-1406 · 248 bp

Back to Cleisostoma

Pi 0.0392

MarkerSeek score 77.50

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 248
variable_sites: 35
pi: 0.0392
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TAGGCTTGTACTTTCGCGTC	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.0	58.6	315-347 bp	1.0	87.0
psbA-trnK-UUU_p2	TAGGCTTGTACTTTCGCGTC	ACTAGTTCCGGGTTCGAGTC	50.0	55.0	58.0	58.8	305-337 bp	1.0	85.3
psbA-trnK-UUU_p3	TAGGCTTGTACTTTCGCGTC	GACTAGTTCCGGGTTCGAGT	50.0	55.0	58.0	58.8	306-338 bp	1.0	85.3
psbA-trnK-UUU_p4	TAGGCTTGTACTTTCGCGTC	ATCCGACTAGTTCCGGGTTC	50.0	55.0	58.0	58.9	310-342 bp	1.0	84.8
psbA-trnK-UUU_p5	AACACCAAACCATCCGATGT	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.7	58.6	381-413 bp	1.0	84.0