Hotspot

ycf2-trnI-CAU

IRa · 153345-153432 · 88 bp

Back to Clausia

Pi 0.0976

MarkerSeek score 75.90

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 88
variable_sites: 8
pi: 0.0976
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	CCAGCGACGGTTTCATTAGA	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	719-725 bp	1.0	82.4
ycf2-trnI-CAU_p2	TCCAGCGACGGTTTCATTAG	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	720-726 bp	1.0	82.4
ycf2-trnI-CAU_p3	CCAGCGACGGTTTCATTAGA	CCCCTCTATGCCTTACGGTA	50.0	55.0	58.0	58.0	1395 bp	0.5	59.9
ycf2-trnI-CAU_p4	TCCAGCGACGGTTTCATTAG	CCCCTCTATGCCTTACGGTA	50.0	55.0	58.0	58.0	1396 bp	0.5	59.9
ycf2-trnI-CAU_p5	CCAGCGACGGTTTCATTAGA	CACTTGACTGTCTACGGCTC	50.0	55.0	58.0	58.0	1301 bp	0.5	59.8