Hotspot

rpl32-trnL-CUA

SSC · 114740-115287 · 548 bp

Back to Clausia

Pi 0.0762

MarkerSeek score 76.70

Primer available yes

Label rpl32-trnL-CUA

Feature Metadata

label_name: rpl32-trnL-CUA
feature_type: igs
region: SSC
length_bp: 548
variable_sites: 41
pi: 0.0762
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-CUA_p1	GCACTCTCATACGGGTTTCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.6	57.9	982 bp	0.5	56.0
rpl32-trnL-CUA_p2	GCACTCTCATACGGGTTTCA	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.6	57.8	975 bp	0.5	55.5
rpl32-trnL-CUA_p3	TGAGCACTCTCATACGGGTT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.4	57.9	985 bp	0.5	55.5
rpl32-trnL-CUA_p4	GCACTCTCATACGGGTTTCA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	57.6	57.8	981 bp	0.5	55.4
rpl32-trnL-CUA_p5	GCACTCTCATACGGGTTTCA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.6	57.8	980 bp	0.5	55.4