Hotspot

trnI-CAU-ycf2

IRb · 85642-85729 · 88 bp

Back to Clausia

Pi 0.0976

MarkerSeek score 75.90

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 88
variable_sites: 8
pi: 0.0976
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	CCAGCGACGGTTTCATTAGA	50.0	50.0	58.0	58.0	719-725 bp	1.0	82.4
trnI-CAU-ycf2_p2	GTTGGGCGCTTTAACCATTC	TCCAGCGACGGTTTCATTAG	50.0	50.0	58.0	58.0	720-726 bp	1.0	82.4
trnI-CAU-ycf2_p3	CACTTGACTGTCTACGGCTC	CCAGCGACGGTTTCATTAGA	55.0	50.0	58.0	58.0	1288-1301 bp	1.0	82.4
trnI-CAU-ycf2_p4	CCCCTCTATGCCTTACGGTA	CCAGCGACGGTTTCATTAGA	55.0	50.0	58.0	58.0	1382-1395 bp	1.0	82.3
trnI-CAU-ycf2_p5	CCCCTCTATGCCTTACGGTA	TCCAGCGACGGTTTCATTAG	55.0	50.0	58.0	58.0	1383-1396 bp	1.0	82.3