Hotspot

ndhC-trnV-GUA

LSC · 49402-50277 · 876 bp

Back to Clausia

Pi 0.044

MarkerSeek score 76.00

Primer available yes

Label ndhC-trnV-GUA

Feature Metadata

label_name: ndhC-trnV-GUA
feature_type: igs
region: LSC
length_bp: 876
variable_sites: 38
pi: 0.044
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-GUA_p1	TGCCAAAACAGGAATAGCACT	TCGAACTGTTTTACCGAGCA	42.9	45.0	58.1	57.5	993-997 bp	1.0	71.0
ndhC-trnV-GUA_p2	TGCCAAAACAGGAATAGCACT	GTTCGAGTCCGTATAGCCCT	42.9	55.0	58.1	58.7	965-969 bp	1.0	69.8
ndhC-trnV-GUA_p3	TGCCAAAACAGGAATAGCAC	TCGAACTGTTTTACCGAGCA	45.0	45.0	56.6	57.5	993-997 bp	1.0	68.8
ndhC-trnV-GUA_p4	TGCCAAAACAGGAATAGCACT	CGAACTGTTTTACCGAGCAG	42.9	50.0	58.1	57.1	992-996 bp	1.0	68.3
ndhC-trnV-GUA_p5	TGCCAAAACAGGAATAGCACT	TTCGAGTCCGTATAGCCCTA	42.9	50.0	58.1	57.1	964-968 bp	1.0	67.7