Hotspot

trnF-UUC-ndhJ

LSC · 47079-47680 · 602 bp

Back to Clausia

Pi 0.0361
MarkerSeek score 76.80
Primer available yes
Label trnF-UUC-ndhJ

Feature Metadata

label_name
trnF-UUC-ndhJ
feature_type
igs
region
LSC
length_bp
602
variable_sites
21
pi
0.0361
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-UUC-ndhJ_p1 CCTCGTGTCACCAGTTCAAA CATCCACGACTGAAACGGAT 50.0 50.0 58.1 58.0 732-741 bp 1.0 84.0
trnF-UUC-ndhJ_p2 CCTCGTGTCACCAGTTCAAA CCCGAAAGTTGGATAGGGTG 50.0 55.0 58.1 58.0 705-714 bp 1.0 83.9
trnF-UUC-ndhJ_p3 CCTCGTGTCACCAGTTCAAA CCGAAAGTTGGATAGGGTGG 50.0 55.0 58.1 58.0 704-713 bp 1.0 83.9
trnF-UUC-ndhJ_p4 CCTCGTGTCACCAGTTCAAA CCACGACTGAAACGGATCTT 50.0 50.0 58.1 57.9 729-738 bp 1.0 83.4
trnF-UUC-ndhJ_p5 CCTCGTGTCACCAGTTCAAA TGATAGCCATCCACGACTGA 50.0 50.0 58.1 58.2 739-748 bp 1.0 82.2