Hotspot

trnS-AGC-trnG-GGA

LSC · 7684-8264 · 581 bp

Back to Clausia

Pi 0.052

MarkerSeek score 77.90

Primer available yes

Label trnS-AGC-trnG-GGA

Feature Metadata

label_name: trnS-AGC-trnG-GGA
feature_type: igs
region: LSC
length_bp: 581
variable_sites: 29
pi: 0.052
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-AGC-trnG-GGA_p1	CCAACGCTTTAGTCCACTCA	TACCACTAAACTATACCCGC	50.0	45.0	57.8	54.0	627-632 bp	1.0	53.2
trnS-AGC-trnG-GGA_p2	AACGCTTTAGTCCACTCAGC	TACCACTAAACTATACCCGC	50.0	45.0	58.2	54.0	625-630 bp	1.0	52.9
trnS-AGC-trnG-GGA_p3	CCAACGCTTTAGTCCACTCA	TTACCACTAAACTATACCCGC	50.0	42.9	57.8	54.9	628-633 bp	1.0	52.1
trnS-AGC-trnG-GGA_p4	AACGCTTTAGTCCACTCAGC	TTACCACTAAACTATACCCGC	50.0	42.9	58.2	54.9	626-631 bp	1.0	51.8
trnS-AGC-trnG-GGA_p5	GCTTTAGTCCACTCAGCCAT	TACCACTAAACTATACCCGC	50.0	45.0	57.6	54.0	622-627 bp	1.0	51.3