Hotspot

trnH-CAC-psbA

LSC · 77-320 · 244 bp

Back to Clausia

Pi 0.125
MarkerSeek score 79.60
Primer available yes
Label trnH-CAC-psbA

Feature Metadata

label_name
trnH-CAC-psbA
feature_type
igs
region
LSC
length_bp
244
variable_sites
30
pi
0.125
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-CAC-psbA_p1 CTGCCTTAATCCACTTGGCT GCTGTTGAGGCTCCATCTAC 50.0 55.0 57.9 58.1 302-303 bp 1.0 87.5
trnH-CAC-psbA_p2 ACTGCCTTAATCCACTTGGC GCTGTTGAGGCTCCATCTAC 50.0 55.0 58.2 58.1 303-304 bp 1.0 87.3
trnH-CAC-psbA_p3 CTGCCTTAATCCACTTGGCT CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 317-318 bp 1.0 87.0
trnH-CAC-psbA_p4 CTGCCTTAATCCACTTGGCT TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 320-321 bp 1.0 86.8
trnH-CAC-psbA_p5 ACTGCCTTAATCCACTTGGC CCTCTAGACCTAGCTGCTGT 50.0 55.0 58.2 57.9 318-319 bp 1.0 86.7