Hotspot

ndhA

SSC · 124034-126260 · 2227 bp

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Pi 0.0217

MarkerSeek score 62.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2227
variable_sites: 105
pi: 0.0217
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCGGTTACCATAGGGAACA	TGTGAGAGTAGAAGCCCCAA	50.0	50.0	58.0	58.0	2503-2532 bp	1.0	84.8
ndhA_p2	ACCGGTTACCATAGGGAACA	AGCCCCAAAAGGAGAATTGG	50.0	50.0	58.0	58.1	2491-2520 bp	1.0	84.6
ndhA_p3	ACCGGTTACCATAGGGAACA	GCTACGAGCGTCTGGAATAG	50.0	55.0	58.0	58.0	2815-2844 bp	1.0	84.4
ndhA_p4	ATTCAATTCGTGACGGTCGT	TGTGAGAGTAGAAGCCCCAA	45.0	50.0	57.9	58.0	2890-2919 bp	1.0	84.1
ndhA_p5	ATTCAATTCGTGACGGTCGT	AGCCCCAAAAGGAGAATTGG	45.0	50.0	57.9	58.1	2878-2907 bp	1.0	83.8