Hotspot

ccsA-ndhD

SSC · 119461-119771 · 311 bp

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Pi 0.0463

MarkerSeek score 78.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 311
variable_sites: 34
pi: 0.0463
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATCCACGGCTCTTCTAGTCA	CTCCCGGCCAACGTAATTTA	50.0	50.0	57.9	58.0	1417-1422 bp	1.0	84.6
ccsA-ndhD_p2	TCCACGGCTCTTCTAGTCAT	CTCCCGGCCAACGTAATTTA	50.0	50.0	57.9	58.0	1416-1421 bp	1.0	84.6
ccsA-ndhD_p3	TGTGGCTTCTATGGGCTTTC	TCTGGACCACGGGAGTTATT	50.0	50.0	57.9	58.1	539-544 bp	0.8	79.3
ccsA-ndhD_p4	TGTGGCTTCTATGGGCTTTC	TTCTGGACCACGGGAGTTAT	50.0	50.0	57.9	58.1	540-545 bp	0.8	79.2
ccsA-ndhD_p5	TGTGGCTTCTATGGGCTTTC	CTCCCGGCCAACGTAATTTA	50.0	50.0	57.9	58.0	1024-1029 bp	0.8	76.4