Hotspot

ndhF-trnL-UAG

SSC · 116682-118324 · 1643 bp

Back to Clausena

Pi 0.0432

MarkerSeek score 78.50

Primer available yes

Label ndhF-trnL-UAG

Feature Metadata

label_name: ndhF-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1643
variable_sites: 152
pi: 0.0432
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnL-UAG_p1	GTTTTGTTGCGGTCGGAAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.1	58.0	1675-2006 bp	1.0	90.2
ndhF-trnL-UAG_p2	GTTTTGTTGCGGTCGGAAAA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.1	58.0	1676-2007 bp	1.0	90.2
ndhF-trnL-UAG_p3	CACTAGCGATGGTCCAAGAC	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.1	58.0	1810-2141 bp	1.0	90.1
ndhF-trnL-UAG_p4	CACTAGCGATGGTCCAAGAC	CAGCGTGTCTACCGATTTCA	55.0	50.0	58.1	58.0	1811-2142 bp	1.0	90.1
ndhF-trnL-UAG_p5	TCACTAGCGATGGTCCAAGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.2	58.0	1811-2142 bp	1.0	89.5