Hotspot

rps3-rps19

IRb · 87118-87632 · 515 bp

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Pi 0.0413

MarkerSeek score 76.00

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: IRb
length_bp: 515
variable_sites: 38
pi: 0.0413
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	CGCCCTGCAATTTGTACTTG	TCCCGGGCATCTACCATTAT	50.0	50.0	58.0	58.0	1191-1410 bp	1.0	85.1
rps3-rps19_p2	TCGGCAAGAATATTGGGGTG	TCCCGGGCATCTACCATTAT	50.0	50.0	57.9	58.0	1077-1296 bp	1.0	85.0
rps3-rps19_p3	GCGCAATTTCTTTTCCGTCA	TCCCGGGCATCTACCATTAT	45.0	50.0	58.0	58.0	1214-1433 bp	1.0	84.8
rps3-rps19_p4	CGCCCTGCAATTTGTACTTG	GCCGCCGTACTAAATAGGAG	50.0	55.0	58.0	57.9	1385-1601 bp	1.0	84.4
rps3-rps19_p5	GCGCAATTTCTTTTCCGTCA	GCCGCCGTACTAAATAGGAG	45.0	55.0	58.0	57.9	1408-1624 bp	1.0	84.1