Hotspot

trnF-GAA-ndhJ

LSC · 51595-51979 · 385 bp

Back to Clausena

Pi 0.0336

MarkerSeek score 72.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 385
variable_sites: 28
pi: 0.0336
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CGTAAGGATTATATTGCCCCCA	50.0	45.5	58.1	57.7	459-470 bp	1.0	69.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ACGTAAGGATTATATTGCCCCC	50.0	45.5	58.1	57.7	460-471 bp	1.0	68.9
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	CGTAAGGATTATATTGCCCCCA	55.0	45.5	58.0	57.7	495-506 bp	1.0	68.8
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	ACGTAAGGATTATATTGCCCCC	55.0	45.5	58.0	57.7	496-507 bp	1.0	68.8
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	CGTAAGGATTATATTGCCCCCA	55.0	45.5	57.9	57.7	483-494 bp	1.0	67.9