Hotspot

petN-psbM

LSC · 29992-31326 · 1335 bp

Back to Clausena

Pi 0.0379

MarkerSeek score 80.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1335
variable_sites: 107
pi: 0.0379
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACATTTTCCCTTTCGCTCGT	CGTTCTAGTTCCTACTGCGT	45.0	50.0	58.1	57.1	1400-1564 bp	1.0	81.7
petN-psbM_p2	CCCTTTCGCTCGTAGTATGG	CGTTCTAGTTCCTACTGCGT	55.0	50.0	57.9	57.1	1393-1557 bp	1.0	81.5
petN-psbM_p3	CCTTTCGCTCGTAGTATGGG	CGTTCTAGTTCCTACTGCGT	55.0	50.0	57.9	57.1	1392-1556 bp	1.0	81.5
petN-psbM_p4	CTTTCGCTCGTAGTATGGGG	CGTTCTAGTTCCTACTGCGT	55.0	50.0	57.9	57.1	1391-1555 bp	1.0	81.5
petN-psbM_p5	TTCGCTCGTAGTATGGGGAA	CGTTCTAGTTCCTACTGCGT	50.0	50.0	58.2	57.1	1389-1553 bp	1.0	80.7