Hotspot

trnR-UCU-atpA

LSC · 10841-11065 · 225 bp

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Pi 0.0484

MarkerSeek score 80.30

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 225
variable_sites: 23
pi: 0.0484
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATGAAAGGCGTCCATTGTCT	TCAGGAACAAAAGGAACGTT	45.0	40.0	57.5	55.4	339-391 bp	1.0	63.9
trnR-UCU-atpA_p2	ATGAAAGGCGTCCATTGTCT	TTCAGGAACAAAAGGAACGT	45.0	40.0	57.5	55.4	340-392 bp	1.0	63.9
trnR-UCU-atpA_p3	GGGAATGAAAGGCGTCCATT	TCAGGAACAAAAGGAACGTT	50.0	40.0	58.5	55.4	343-395 bp	1.0	63.6
trnR-UCU-atpA_p4	GGGAATGAAAGGCGTCCATT	TTCAGGAACAAAAGGAACGT	50.0	40.0	58.5	55.4	344-396 bp	1.0	63.6
trnR-UCU-atpA_p5	AATGGGAATGAAAGGCGTCC	TCAGGAACAAAAGGAACGTT	50.0	40.0	58.5	55.4	346-398 bp	1.0	63.5