Hotspot

trnS-GCU-trnS-CGA

LSC · 8905-9771 · 867 bp

Back to Clausena

Pi 0.0322

MarkerSeek score 75.50

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 867
variable_sites: 63
pi: 0.0322
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	CCGACGCTTTAATCCACTCA	TGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.6	895-935 bp	1.0	62.2
trnS-GCU-trnS-CGA_p2	CCGACGCTTTAATCCACTCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	892-932 bp	1.0	61.2
trnS-GCU-trnS-CGA_p3	ACGGAAAGAGAGGGATTCGA	TGAACGAATCACACTTTTACCAC	50.0	39.1	58.2	57.6	953-993 bp	1.0	60.7
trnS-GCU-trnS-CGA_p4	AACGAAACGGAAAGAGAGGG	TGAACGAATCACACTTTTACCAC	50.0	39.1	57.8	57.6	959-999 bp	1.0	60.5
trnS-GCU-trnS-CGA_p5	CCGACGCTTTAATCCACTCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	894-934 bp	1.0	60.1