Hotspot

ndhF-rpl32

SSC · 102083-102644 · 562 bp

Back to Cladopus

Pi 0.0278

MarkerSeek score 67.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 562
variable_sites: 28
pi: 0.0278
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGTCGTGTAAACCAAAACCC	GTACGTTTCTTTGGCACTGC	50.0	50.0	57.2	58.0	1066-1099 bp	1.0	78.7
ndhF-rpl32_p2	CCCCCGGAAAAAGGAGAATC	GTACGTTTCTTTGGCACTGC	55.0	50.0	58.2	58.0	654-679 bp	0.75	73.5
ndhF-rpl32_p3	CCCCCGGAAAAAGGAGAATC	CCAGTGTTACTGCGATCCTT	55.0	50.0	58.2	57.6	1344-1369 bp	0.75	68.3
ndhF-rpl32_p4	CCCCCGGAAAAAGGAGAATC	TCCAGTGTTACTGCGATCCT	55.0	50.0	58.2	58.4	1345-1370 bp	0.75	67.9
ndhF-rpl32_p5	CCCCCGGAAAAAGGAGAATC	ATCCAGTGTTACTGCGATCC	55.0	50.0	58.2	57.4	1346-1371 bp	0.75	66.6