Hotspot

trnP-UGG-psaJ

LSC · 62426-62956 · 531 bp

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Pi 0.0138

MarkerSeek score 60.60

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 531
variable_sites: 12
pi: 0.0138
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	CGGGTGCAACAGATAGATAGG	50.0	52.4	58.1	58.0	597-653 bp	1.0	75.8
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	CGGGTGCAACAGATAGATAGG	50.0	52.4	58.1	58.0	596-652 bp	1.0	75.8
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	CGGGTGCAACAGATAGATAGG	50.0	52.4	58.1	58.0	608-664 bp	1.0	75.7
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	CGGGTGCAACAGATAGATAGG	50.0	52.4	58.1	58.0	607-663 bp	1.0	75.7
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	CGGGTGCAACAGATAGATAGG	50.0	52.4	58.1	58.0	610-666 bp	1.0	75.7