Hotspot

trnQ-UUG-trnK-UUU

LSC · 49753-50457 · 705 bp

Back to Cladopus

Pi 0.0255
MarkerSeek score 79.70
Primer available yes
Label trnQ-UUG-trnK-UUU

Feature Metadata

label_name
trnQ-UUG-trnK-UUU
feature_type
igs
region
LSC
length_bp
705
variable_sites
34
pi
0.0255
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-trnK-UUU_p1 TAAGGCAACGGGTTTTGGTC AAAGCCGAGTACTCTACCGT 50.0 50.0 58.7 58.2 765-793 bp 1.0 80.1
trnQ-UUG-trnK-UUU_p2 TAAGGCAACGGGTTTTGGTC AAGCCGAGTACTCTACCGTT 50.0 50.0 58.7 58.2 764-792 bp 1.0 80.1
trnQ-UUG-trnK-UUU_p3 GTAAGGCAACGGGTTTTGGT AAAGCCGAGTACTCTACCGT 50.0 50.0 59.0 58.2 766-794 bp 1.0 77.8
trnQ-UUG-trnK-UUU_p4 GTAAGGCAACGGGTTTTGGT AAGCCGAGTACTCTACCGTT 50.0 50.0 59.0 58.2 765-793 bp 1.0 77.8
trnQ-UUG-trnK-UUU_p5 GTGGTAAGGCAACGGGTTTT AAGCCGAGTACTCTACCGTT 50.0 50.0 59.0 58.2 768-796 bp 1.0 77.8