Hotspot

trnR-UCU-trnG-UCC

LSC · 46423-46810 · 388 bp

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Pi 0.0412

MarkerSeek score 79.40

Primer available yes

Label trnR-UCU-trnG-UCC

Feature Metadata

label_name: trnR-UCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 388
variable_sites: 29
pi: 0.0412
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnG-UCC_p1	GAATAGATCCCGCGTCCAAT	AGCCTTCCAAGCTAACGATG	50.0	50.0	57.5	58.0	509-518 bp	1.0	81.9
trnR-UCU-trnG-UCC_p2	GAATAGATCCCGCGTCCAAT	CCTAGCCTTCCAAGCTAACG	50.0	55.0	57.5	58.1	512-521 bp	1.0	81.6
trnR-UCU-trnG-UCC_p3	ATGAATAGATCCCGCGTCCA	AGCCTTCCAAGCTAACGATG	50.0	50.0	58.7	58.0	511-520 bp	1.0	80.2
trnR-UCU-trnG-UCC_p4	ATGAATAGATCCCGCGTCCA	CCTAGCCTTCCAAGCTAACG	50.0	55.0	58.7	58.1	514-523 bp	1.0	79.9
trnR-UCU-trnG-UCC_p5	TAGATCCCGCGTCCAATAGG	AGCCTTCCAAGCTAACGATG	55.0	50.0	58.7	58.0	506-515 bp	1.0	79.6