Hotspot

atpH-atpF

LSC · 42598-43228 · 631 bp

Back to Cladopus

Pi 0.013

MarkerSeek score 68.70

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 631
variable_sites: 16
pi: 0.013
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	AAGCGGAAGGAAAGATACGG	CAGTGACCCAAGAGAACGAA	50.0	50.0	57.7	57.5	783-789 bp	1.0	75.5
atpH-atpF_p2	ACTGGTTGTAGCATTAGCCC	CAGTGACCCAAGAGAACGAA	50.0	50.0	57.6	57.5	713-719 bp	1.0	74.9
atpH-atpF_p3	CGGAAGCGGAAGGAAAGATA	CAGTGACCCAAGAGAACGAA	50.0	50.0	57.4	57.5	786-792 bp	1.0	73.2
atpH-atpF_p4	CTGGTTGTAGCATTAGCCCT	CAGTGACCCAAGAGAACGAA	50.0	50.0	57.3	57.5	712-718 bp	1.0	72.5
atpH-atpF_p5	AAGCGGAAGGAAAGATACGG	GCCAGTGACCCAAGAGAAC	50.0	57.9	57.7	58.1	785-791 bp	1.0	71.2