Hotspot

trnC-GCA-rpoB

LSC · 28361-29277 · 917 bp

Back to Cladopus

Pi 0.0113

MarkerSeek score 59.60

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 917
variable_sites: 20
pi: 0.0113
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAAGGATTTGCAGTCCCCC	CCTTGACCAATGAACCGACA	50.0	50.0	58.4	58.1	1039-1051 bp	1.0	78.7
trnC-GCA-rpoB_p2	TTGTTAAATAGGCGGCACCC	CCTTGACCAATGAACCGACA	50.0	50.0	58.5	58.1	1075-1087 bp	1.0	77.1
trnC-GCA-rpoB_p3	TAAATAGGCGGCACCCAGAT	CCTTGACCAATGAACCGACA	50.0	50.0	58.9	58.1	1071-1083 bp	1.0	74.5
trnC-GCA-rpoB_p4	GTTAAATAGGCGGCACCCAG	CCTTGACCAATGAACCGACA	55.0	50.0	59.0	58.1	1073-1085 bp	1.0	73.6
trnC-GCA-rpoB_p5	CGGCACCCAGATTTGAACT	CCTTGACCAATGAACCGACA	52.6	50.0	58.1	58.1	1063-1075 bp	1.0	73.0