Hotspot

trnY-GUA-trnD-GUC

LSC · 25794-26219 · 426 bp

Back to Cladopus

Pi 0.0145
MarkerSeek score 61.90
Primer available yes
Label trnY-GUA-trnD-GUC

Feature Metadata

label_name
trnY-GUA-trnD-GUC
feature_type
igs
region
LSC
length_bp
426
variable_sites
12
pi
0.0145
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnD-GUC_p1 GGATTCTTCCTGGGTCGATG CGGTGCTCTGACCAATTGAA 55.0 50.0 57.8 58.5 551-560 bp 1.0 76.5
trnY-GUA-trnD-GUC_p2 TTGGCACTATGTCTACGCTG CGGTGCTCTGACCAATTGAA 50.0 50.0 57.7 58.5 497-506 bp 1.0 76.4
trnY-GUA-trnD-GUC_p3 GCACTATGTCTACGCTGGTT CGGTGCTCTGACCAATTGAA 50.0 50.0 57.7 58.5 494-503 bp 1.0 76.4
trnY-GUA-trnD-GUC_p4 GTTGGCACTATGTCTACGCT CGGTGCTCTGACCAATTGAA 50.0 50.0 57.7 58.5 498-507 bp 1.0 76.4
trnY-GUA-trnD-GUC_p5 GGGGACGGACTGTAAATTCG CGGTGCTCTGACCAATTGAA 55.0 50.0 58.4 58.5 517-526 bp 1.0 75.8