Hotspot

trnT-UGU-rps4

LSC · 11069-11534 · 466 bp

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Pi 0.0165
MarkerSeek score 60.80
Primer available yes
Label trnT-UGU-rps4

Feature Metadata

label_name
trnT-UGU-rps4
feature_type
igs
region
LSC
length_bp
466
variable_sites
14
pi
0.0165
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-rps4_p1 TAAAAGCCCGCTTAGCTCAG GAAAAGAGGCCCTCGGTAAC 50.0 55.0 58.0 58.3 542-571 bp 1.0 83.7
trnT-UGU-rps4_p2 CTCAGAGGTTAGAGCATCGC GAAAAGAGGCCCTCGGTAAC 55.0 55.0 57.9 58.3 527-556 bp 1.0 82.9
trnT-UGU-rps4_p3 GATGGTCATCGGTTCGACTC GAAAAGAGGCCCTCGGTAAC 55.0 55.0 58.2 58.3 496-525 bp 1.0 82.8
trnT-UGU-rps4_p4 TAAAAGCCCGCTTAGCTCAG TTTTGAAAAGAGGCCCTCGG 50.0 50.0 58.0 58.4 546-575 bp 1.0 82.7
trnT-UGU-rps4_p5 CTCAGAGGTTAGAGCATCGC TTTTGAAAAGAGGCCCTCGG 55.0 50.0 57.9 58.4 531-560 bp 1.0 81.9