Hotspot

ndhJ-trnF-GAA

LSC · 9513-9991 · 479 bp

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Pi 0.0146

MarkerSeek score 62.10

Primer available yes

Label ndhJ-trnF-GAA

Feature Metadata

label_name: ndhJ-trnF-GAA
feature_type: igs
region: LSC
length_bp: 479
variable_sites: 13
pi: 0.0146
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnF-GAA_p1	TAAGCGGATCTTAATGCCGG	AACCCCATCTTGCAAATCGT	50.0	45.0	57.8	58.1	664-691 bp	1.0	80.4
ndhJ-trnF-GAA_p2	ATCATCCACGACTTAAGCGG	AACCCCATCTTGCAAATCGT	50.0	45.0	57.8	58.1	677-704 bp	1.0	79.9
ndhJ-trnF-GAA_p3	CATCCACGACTTAAGCGGAT	AACCCCATCTTGCAAATCGT	50.0	45.0	57.8	58.1	675-702 bp	1.0	79.9
ndhJ-trnF-GAA_p4	CCACGACTTAAGCGGATCTT	AACCCCATCTTGCAAATCGT	50.0	45.0	57.7	58.1	672-699 bp	1.0	79.3
ndhJ-trnF-GAA_p5	ATGCCGGAAAGTTGGATAGG	AACCCCATCTTGCAAATCGT	50.0	45.0	57.7	58.1	651-678 bp	1.0	79.0