Hotspot

rbcL-atpB

LSC · 3541-4522 · 982 bp

Back to Cladopus

Pi 0.0195

MarkerSeek score 58.40

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: LSC
length_bp: 982
variable_sites: 37
pi: 0.0195
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	GTCCACACAGTTGTCCATGT	TCTAATTGGGTAAACGCGGG	50.0	50.0	58.0	58.0	1618-1640 bp	1.0	82.0
rbcL-atpB_p2	GCCTTGAATCCAACACTTGC	TCTAATTGGGTAAACGCGGG	50.0	50.0	57.9	58.0	1450-1472 bp	1.0	82.0
rbcL-atpB_p3	TACAGCCTAATAGGGGACGG	TCTAATTGGGTAAACGCGGG	55.0	50.0	58.0	58.0	1923-1945 bp	1.0	81.8
rbcL-atpB_p4	CCTAATAGGGGACGGCCATA	TCTAATTGGGTAAACGCGGG	55.0	50.0	58.1	58.0	1918-1940 bp	1.0	81.4
rbcL-atpB_p5	AGGCGGTCCTTGAAAAGTTT	TCTAATTGGGTAAACGCGGG	45.0	50.0	57.9	58.0	1862-1884 bp	1.0	81.4