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psbA-rbcL

LSC · 1439-2112 · 674 bp

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Pi 0.0287

MarkerSeek score 76.50

Primer available yes

Label psbA-rbcL

Feature Metadata

label_name: psbA-rbcL
feature_type: igs
region: LSC
length_bp: 674
variable_sites: 34
pi: 0.0287
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-rbcL_p1	TACAGAAGCGACCCCATAGG	AAGTCATTCGTGAAGCGTGT	55.0	45.0	58.6	58.1	792-830 bp	1.0	81.5
psbA-rbcL_p2	TACAGAAGCGACCCCATAGG	TGAAGTCATTCGTGAAGCGT	55.0	45.0	58.6	57.9	794-832 bp	1.0	81.4
psbA-rbcL_p3	ATCCAGTTACAGAAGCGACC	AAGTCATTCGTGAAGCGTGT	50.0	45.0	57.3	58.1	799-837 bp	1.0	80.8
psbA-rbcL_p4	ATCCAGTTACAGAAGCGACC	TGAAGTCATTCGTGAAGCGT	50.0	45.0	57.3	57.9	801-839 bp	1.0	80.7
psbA-rbcL_p5	AGTTACAGAAGCGACCCCAT	AAGTCATTCGTGAAGCGTGT	50.0	45.0	58.7	58.1	795-833 bp	1.0	80.4