Hotspot

trnH-GUG-psbA

LSC · 68-376 · 309 bp

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Pi 0.0599

MarkerSeek score 78.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 309
variable_sites: 34
pi: 0.0599
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TTGCCTTAATCCACTTGGCT	GCGCTAACCTTGGTATGGAA	45.0	50.0	57.4	58.0	429-487 bp	1.0	84.9
trnH-GUG-psbA_p2	AACCCGCGAATGATGGATTC	GCGCTAACCTTGGTATGGAA	50.0	50.0	58.7	58.0	457-515 bp	1.0	83.6
trnH-GUG-psbA_p3	GAACCCGCGAATGATGGATT	GCGCTAACCTTGGTATGGAA	50.0	50.0	58.7	58.0	458-516 bp	1.0	83.6
trnH-GUG-psbA_p4	CCGCGAATGATGGATTCACA	GCGCTAACCTTGGTATGGAA	50.0	50.0	58.7	58.0	454-512 bp	1.0	83.6
trnH-GUG-psbA_p5	TTGCCTTAATCCACTTGGCT	GTGTATTCGGCGGCTCTTTA	45.0	50.0	57.4	58.1	786-844 bp	1.0	80.3