Hotspot

rps4

LSC · 47229-47834 · 606 bp

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Pi 0.00165

MarkerSeek score 59.70

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: LSC
length_bp: 606
variable_sites: 1
pi: 0.00165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	ATACGTGAATGAGACAGGGC	TGAGTTTTGCTTTGCTGATCC	50.0	42.9	57.4	57.4	783 bp	1.0	62.1
rps4_p2	TACGTGAATGAGACAGGGCA	TGAGTTTTGCTTTGCTGATCC	50.0	42.9	58.7	57.4	782 bp	1.0	61.0
rps4_p3	ATACGTGAATGAGACAGGGC	GAGTTTTGCTTTGCTGATCCT	50.0	42.9	57.4	57.1	782 bp	1.0	59.8
rps4_p4	ACTTCAGCGAAAGGAGTCAA	TGAGTTTTGCTTTGCTGATCC	45.0	42.9	57.1	57.4	747 bp	1.0	59.6
rps4_p5	ATACGTGAATGAGACAGGGC	TTGAGTTTTGCTTTGCTGATCC	50.0	40.9	57.4	58.0	784 bp	1.0	59.1