Hotspot

rps2

LSC · 16173-16892 · 720 bp

Back to Claderia

Pi 0.00139

MarkerSeek score 59.50

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 720
variable_sites: 1
pi: 0.00139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	ACTCCAAAAGATGCAAACCA	ATCCTGGTAGAAGGTTCCGT	40.0	50.0	55.7	57.8	914 bp	1.0	60.0
rps2_p2	ACTCCAAAAGATGCAAACCA	TCCTGGTAGAAGGTTCCGTC	40.0	55.0	55.7	58.4	913 bp	1.0	58.4
rps2_p3	AACTCCAAAAGATGCAAACCA	ATCCTGGTAGAAGGTTCCGT	38.1	50.0	56.5	57.8	915 bp	1.0	58.3
rps2_p4	ACTCCAAAAGATGCAAACCAA	ATCCTGGTAGAAGGTTCCGT	38.1	50.0	56.5	57.8	914 bp	1.0	58.3
rps2_p5	ACTCCAAAAGATGCAAACCAA	TCCTGGTAGAAGGTTCCGTC	38.1	55.0	56.5	58.4	913 bp	1.0	56.8