Hotspot

rps19

IRb · 88503-88781 · 279 bp

Back to Claderia

Pi 0.00717

MarkerSeek score 56.60

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: IRb
length_bp: 279
variable_sites: 2
pi: 0.00717
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	AGGCTTTTCACATGAATACT	CGAGATATTGGACATAGAATGCC	35.0	43.5	52.4	57.0	367 bp	1.0	40.4
rps19_p2	AAGGCTTTTCACATGAATACT	CGAGATATTGGACATAGAATGCC	33.3	43.5	53.4	57.0	368 bp	1.0	40.4
rps19_p3	AGGCTTTTCACATGAATACTT	CGAGATATTGGACATAGAATGCC	33.3	43.5	53.4	57.0	367 bp	1.0	40.4
rps19_p4	AGGCTTTTCACATGAATACTTAGT	CGAGATATTGGACATAGAATGCC	33.3	43.5	56.4	57.0	367 bp	1.0	40.4
rps19_p5	AAGGCTTTTCACATGAATACTT	CGAGATATTGGACATAGAATGCC	31.8	43.5	54.2	57.0	368 bp	1.0	40.4