Hotspot

psbD

LSC · 34849-35910 · 1062 bp

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Pi 0.000942

MarkerSeek score 59.30

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	AATCGAACTACCACATGCCC	GAGCCCCAAGTAGTTTACCG	50.0	55.0	58.0	58.0	1242 bp	1.0	79.6
psbD_p2	CACATGCCCTGGAAATGAGA	GAGCCCCAAGTAGTTTACCG	50.0	55.0	57.9	58.0	1231 bp	1.0	78.8
psbD_p3	TACCACATGCCCTGGAAATG	GAGCCCCAAGTAGTTTACCG	50.0	55.0	57.9	58.0	1234 bp	1.0	78.8
psbD_p4	AATCGAACTACCACATGCCC	TCACGACCGACTAAGGCTAA	50.0	50.0	58.0	58.2	1165 bp	1.0	78.4
psbD_p5	AATCGAACTACCACATGCCC	GATAAGTCTCGCATTCCCGG	50.0	55.0	58.0	58.2	1214 bp	1.0	78.0