Hotspot

psbC

LSC · 35858-37279 · 1422 bp

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Pi 0.000703

MarkerSeek score 59.20

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 1
pi: 0.000703
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TTTCCCAAGAAATCCGTGCA	TACTCGTTTGGACTGTCCCT	45.0	50.0	58.0	58.0	1652 bp	1.0	79.9
psbC_p2	TTTCCCAAGAAATCCGTGCA	CTCGTTTGGACTGTCCCTTT	45.0	50.0	58.0	57.7	1650 bp	1.0	77.9
psbC_p3	GCAGCGGAAGATCCTGAATT	TACTCGTTTGGACTGTCCCT	50.0	50.0	58.3	58.0	1635 bp	1.0	77.4
psbC_p4	AGGTATTCGTGCTTGGATGG	TACTCGTTTGGACTGTCCCT	50.0	50.0	57.7	58.0	1579 bp	1.0	77.4
psbC_p5	GTTTCCCAAGAAATCCGTGC	TACTCGTTTGGACTGTCCCT	50.0	50.0	57.7	58.0	1653 bp	1.0	77.2