Hotspot

ndhK

LSC · 51577-52350 · 774 bp

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Pi 0.00129

MarkerSeek score 59.50

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 774
variable_sites: 1
pi: 0.00129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	AATGATTGACCCGCTGTTTC	TGGGCAATGAGCTTTGATGT	45.0	45.0	56.7	58.1	904 bp	1.0	69.1
ndhK_p2	AATGATTGACCCGCTGTTTC	ATCCATGGGCAATGAGCTTT	45.0	45.0	56.7	57.8	909 bp	1.0	68.3
ndhK_p3	AATGATTGACCCGCTGTTTC	GGTTCAGTTTATGCATGGCG	45.0	50.0	56.7	57.8	829 bp	1.0	68.1
ndhK_p4	AATGATTGACCCGCTGTTTC	GGGTTCAGTTTATGCATGGC	45.0	50.0	56.7	57.4	830 bp	1.0	65.0
ndhK_p5	GCATCTTCTTCATCTCTCCCA	TGGGCAATGAGCTTTGATGT	47.6	45.0	57.2	58.1	969 bp	1.0	64.8