Hotspot

ndhH

SSC · 127366-128547 · 1182 bp

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Pi 0.000846

MarkerSeek score 59.20

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 1
pi: 0.000846
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	ATCCCATCCGCTAAAGCTTG	TATCGCAAAGGGGTCTACGA	50.0	50.0	58.0	58.2	1710 bp	1.0	78.0
ndhH_p2	TGTTCCAACGAATCACACGT	TATCGCAAAGGGGTCTACGA	45.0	50.0	58.1	58.2	2042 bp	1.0	77.7
ndhH_p3	ATCCCATCCGCTAAAGCTTG	TTCTGGGAAAACGTCAACGA	50.0	45.0	58.0	57.7	1686 bp	1.0	77.3
ndhH_p4	TGTTCCAACGAATCACACGT	TTCTGGGAAAACGTCAACGA	45.0	45.0	58.1	57.7	2018 bp	1.0	77.1
ndhH_p5	CCCATCCGCTAAAGCTTGAA	TATCGCAAAGGGGTCTACGA	50.0	50.0	58.2	58.2	1708 bp	1.0	76.2