Hotspot

atpE

LSC · 54411-54815 · 405 bp

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Pi 0.00494

MarkerSeek score 57.50

Primer available yes

Label atpE

Feature Metadata

label_name: atpE
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 2
pi: 0.00494
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE_p1	ACTTCATTGTGTTCAATTTGTGGA	TTAGACAGTCTTCCCGAGCA	33.3	50.0	57.8	58.1	591 bp	1.0	45.9
atpE_p2	GACTTCATTGTGTTCAATTTGTGG	TTAGACAGTCTTCCCGAGCA	37.5	50.0	57.5	58.1	592 bp	1.0	43.5
atpE_p3	ACTTCATTGTGTTCAATTTGTGG	TTAGACAGTCTTCCCGAGCA	34.8	50.0	56.4	58.1	591 bp	1.0	43.1
atpE_p4	ACTTCATTGTGTTCAATTTGTGGA	AACATCGATGAAGCTACCGC	33.3	50.0	57.8	58.4	552 bp	1.0	43.1
atpE_p5	ACTTCATTGTGTTCAATTTGTGGA	CCCGAGCAGGCCTTTTATTT	33.3	50.0	57.8	58.5	579 bp	1.0	42.3