Hotspot

trnN-GUU-ndhF

IRb · 113916-115246 · 1331 bp

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Pi 0
MarkerSeek score 25.10
Primer available yes
Label trnN-GUU-ndhF

Feature Metadata

label_name
trnN-GUU-ndhF
feature_type
igs
region
IRb
length_bp
1331
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
25.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ndhF_p1 GGCAGAATAGCAGAGCAAGT ATGGGATTGGTGTTGCAAGT 50.0 45.0 58.0 58.0 1902 bp 1.0 79.7
trnN-GUU-ndhF_p2 GGCAGAATAGCAGAGCAAGT AATATATGGGAGGTGGGCGA 50.0 50.0 58.0 58.0 1860 bp 1.0 79.5
trnN-GUU-ndhF_p3 GGCAGAATAGCAGAGCAAGT ATATATGGGAGGTGGGCGAA 50.0 50.0 58.0 58.0 1859 bp 1.0 79.5
trnN-GUU-ndhF_p4 GGCAGAATAGCAGAGCAAGT TATATGGGAGGTGGGCGAAT 50.0 50.0 58.0 58.0 1858 bp 1.0 79.5
trnN-GUU-ndhF_p5 AAGCGAGCCCTTTCATTCAA ATGGGATTGGTGTTGCAAGT 45.0 45.0 58.1 58.0 1619 bp 1.0 79.3