Hotspot

atpI-rps2

LSC · 15881-16172 · 292 bp

Back to Claderia

Pi 0
MarkerSeek score 18.80
Primer available yes
Label atpI-rps2

Feature Metadata

label_name
atpI-rps2
feature_type
igs
region
LSC
length_bp
292
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
18.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-rps2_p1 TATATCATTCGTGGGCGCAG AAGCGGGAGTTCATTTTGGT 50.0 45.0 57.9 58.0 1371 bp 1.0 79.4
atpI-rps2_p2 TATATCATTCGTGGGCGCAG GCAATTTGTGAGGGCCATTC 50.0 50.0 57.9 58.0 745 bp 1.0 79.4
atpI-rps2_p3 TATATCATTCGTGGGCGCAG ATCAGCAGCAATAAGGGCTC 50.0 50.0 57.9 58.0 1160 bp 1.0 79.3
atpI-rps2_p4 TATATCATTCGTGGGCGCAG CAGCAATAAGGGCTCGATGT 50.0 50.0 57.9 58.0 1155 bp 1.0 79.1
atpI-rps2_p5 TATATCATTCGTGGGCGCAG GATGGAAGCGGGAGTTCATT 50.0 50.0 57.9 58.0 1376 bp 1.0 79.1