Hotspot

atpH-atpI

LSC · 14403-15136 · 734 bp

Back to Claderia

Pi 0

MarkerSeek score 25.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCCACGACCAGTCCATAAAT	CTGCGCCCACGAATGATATA	50.0	50.0	58.0	57.9	1308 bp	1.0	79.1
atpH-atpI_p2	CGCAATACCTTCTACGGCTT	CTGCGCCCACGAATGATATA	50.0	50.0	58.1	57.9	1216 bp	1.0	78.9
atpH-atpI_p3	TAAACTCGAAACTCCCAGCG	CTGCGCCCACGAATGATATA	50.0	50.0	57.9	57.9	1571 bp	1.0	78.8
atpH-atpI_p4	GCCACGACCAGTCCATAAAT	AACTTTGGCTGCGGCTTATA	50.0	45.0	58.0	57.9	995 bp	1.0	78.8
atpH-atpI_p5	ATTGTGCACCCTTTGTTCCT	CTGCGCCCACGAATGATATA	45.0	50.0	57.9	57.9	1755 bp	1.0	78.7