Hotspot

rpl22

LSC · 87428-87919 · 492 bp

Pi 0.0154

MarkerSeek score 47.80

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 492
variable_sites: 30
pi: 0.0154
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	GTACCAAGCCGGAAACCTAG	TCCCGGGCATCTACCATTAT	55.0	50.0	58.0	58.0	731-773 bp	1.0	84.5
rpl22_p2	GTACCAAGCCGGAAACCTAG	GCCGCCGTACTAAATAGGAG	55.0	55.0	58.0	57.9	923-966 bp	1.0	83.5
rpl22_p3	CGCCCTGCAATTTGTACTTG	TCCCGGGCATCTACCATTAT	50.0	50.0	58.0	58.0	1190-1232 bp	1.0	83.1
rpl22_p4	GCGCAATTTCTTTTCCGTCA	TCCCGGGCATCTACCATTAT	45.0	50.0	58.0	58.0	1213-1255 bp	1.0	82.8
rpl22_p5	CGCCCTGCAATTTGTACTTG	GCCGCCGTACTAAATAGGAG	50.0	55.0	58.0	57.9	1382-1425 bp	1.0	82.6